MyBioSource.com's MOUSE ANTI HUMAN TROPONIN I (CARDIAC/PHOSPHORYLATED) is a Mouse monoclonal antibody. The MOUSE ANTI HUMAN TROPONIN I (CARDIAC/PHOSPHORYLATED) Antibody was generated using cardiac troponin I, cTnI, TNI, and TNNI3 as the antigen. It reacts with Rat, Bovine, Canine, Feline, Goat, and Porcine. This antibody has been shown to work in applications such as: ELISA, and Western Blot.
Description
Mouse anti Human Troponin I (Cardiac/Phosphorylated) antibody, clone 1G11 recognizes the phosphorylated form of human cardiac Troponin I (cTnI). Troponin I is a 24kDa subunit of Troponin, a protein found on muscle tissue responsible for muscle contraction. When calcium binds to Troponin C, a conformational change moves Troponin I and tropomyosin out of the way of the myosin binding sites on the actin filaments in muscles. This allows cross-bridging to occur and the muscle to contract. Levels of cTnI in serum are commonly used as a marker for cardiac cell damage and death. Phosphorylation of cTnI results in changes in the activity and function of Troponin. One possible form of phosphorylation is by Protein kinase A. Protein kinase A phosphorylation of serine 22 and serine 23 reduces calcium sensitivity of Troponin and leads to an acceleration in relaxation. Changes in cTnI phosphorylation have been reported in failing hearts. Mouse anti Human Troponin I (Cardiac/Phosphorylated) antibody, clone 1G11 recognizes free cTnI and cTnI in complex with other Troponin components. It does not cross-react with skeletal Troponin I